Journal: The World Allergy Organization Journal

Article Title: Increased glucose transporter 1 contributes to epithelial barrier dysfunction in allergic rhinitis ☆

doi: 10.1016/j.waojou.2025.101158

Figure Lengend Snippet: GLUT1 is elevated in the nasal mucosa and positively correlated with disease severity in AR patients. (A–B) Immunofluorescence staining for GLUT1 (n = 12); (C–D) WB showing GLUT1 protein expression (n = 12); (E) RT-qPCR for GLUT1 (n = 30); (F–G) The correlations between GLUT1 mRNA, VAS and TNSS in AR patients (n = 30). AR, allergic rhinitis; HCs, healthy control; GLUT1, glucose transporter 1; WB, western blotting; RT-qPCR, quantitative reverse transcription polymerase chain reaction; VAS, visual analog scale; TNSS, total nasal symptom score. ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

Article Snippet: Slides were then incubated overnight at 4 °C with an anti-GLUT1 primary antibody (1:200, Proteintech, Wuhan, China).

Techniques: Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Control, Western Blot, Reverse Transcription, Polymerase Chain Reaction

Journal: The World Allergy Organization Journal

Article Title: Increased glucose transporter 1 contributes to epithelial barrier dysfunction in allergic rhinitis ☆

doi: 10.1016/j.waojou.2025.101158

Figure Lengend Snippet: Increased GLUT1 is associated with epithelial tight junction marker expression in AR patients. (A–B) Tissue multiplex immunofluorescence for GLUT1, ZO-1, and occluding; (C–D) RT-qPCR for ZO-1 and occluding between the 2 groups (n = 30); (E–F) The correlations between GLUT1 mRNA, ZO-1 and occluding mRNA in AR patients (n = 30). AR, allergic rhinitis; HCs, healthy control; GLUT1, glucose transporter 1; RT-qPCR, quantitative reverse transcription polymerase chain reaction. ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: Slides were then incubated overnight at 4 °C with an anti-GLUT1 primary antibody (1:200, Proteintech, Wuhan, China).

Techniques: Marker, Expressing, Multiplex Assay, Immunofluorescence, Quantitative RT-PCR, Control, Reverse Transcription, Polymerase Chain Reaction

Journal: The World Allergy Organization Journal

Article Title: Increased glucose transporter 1 contributes to epithelial barrier dysfunction in allergic rhinitis ☆

doi: 10.1016/j.waojou.2025.101158

Figure Lengend Snippet: HDM stimulation promotes GLUT1 expression and inhibits tight junction marker expression in nasal epithelial cells. (A–B) Cell immunofluorescence staining for GLUT1, ZO-1 and occluding in nasal epithelial cells treated with different concentrations of HDM (n = 6); (C–D) WB showing GLUT1, ZO-1 and occluding protein expression in nasal epithelial cells treated with different concentrations of HDM (n = 6). HDM, house dust mite; GLUT1, glucose transporter 1; WB, western blotting. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

Article Snippet: Slides were then incubated overnight at 4 °C with an anti-GLUT1 primary antibody (1:200, Proteintech, Wuhan, China).

Techniques: Expressing, Marker, Immunofluorescence, Staining, Western Blot

Journal: The World Allergy Organization Journal

Article Title: Increased glucose transporter 1 contributes to epithelial barrier dysfunction in allergic rhinitis ☆

doi: 10.1016/j.waojou.2025.101158

Figure Lengend Snippet: Inhibiting GLUT1 alleviates the suppression of tight junction marker expression in nasal epithelial cells mediated by HDM. (A–B) Cell immunofluorescence staining for GLUT1, ZO-1 and occluding in nasal epithelial cells treated with PBS, HDM and HDM + BAY876 (n = 6); WB showing GLUT1, ZO-1 and occluding protein expression in nasal epithelial cells among the 3 groups (n = 6). HDM, house dust mite; GLUT1, glucose transporter 1; WB, western blotting. ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

Article Snippet: Slides were then incubated overnight at 4 °C with an anti-GLUT1 primary antibody (1:200, Proteintech, Wuhan, China).

Techniques: Marker, Expressing, Immunofluorescence, Staining, Western Blot

Journal: The World Allergy Organization Journal

Article Title: Increased glucose transporter 1 contributes to epithelial barrier dysfunction in allergic rhinitis ☆

doi: 10.1016/j.waojou.2025.101158

Figure Lengend Snippet: GLUT1 inhibitor alleviates allergen-mediated nasal mucosal inflammation and barrier function damage in murine AR model. (A) Representative HE and immunofluorescence images of nasal mucosal in PBS, AR and AR + BAY876 groups (n = 5); (B) Relative fluorescence intensity of GLUT1, ZO-1 and occluding among the 3 groups (n = 5); (C) Relative fluorescence intensity of GLUT1, ZO-1 and occluding among the 3 groups (n = 5); (D) IL-4, IL-13, IL-17A and IFN-γ concentrations in nasal lavage fluid (n = 5). AR, allergic rhinitis; GLUT1, glucose transporter 1; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: Slides were then incubated overnight at 4 °C with an anti-GLUT1 primary antibody (1:200, Proteintech, Wuhan, China).

Techniques: Immunofluorescence, Fluorescence

Journal: CNS Neuroscience & Therapeutics

Article Title: Electroacupuncture Prevents Against AD‐Like Phenotypes in APP/PS1 Mice: Investigation of the Mechanisms From Cerebral Microangiopathy

doi: 10.1002/cns.70696

Figure Lengend Snippet: Electroacupuncture inhibits abnormal expression of microvessels and inflammation‐related proteins in APP/PS1 mice. (A) Representative immunoblots of PDGFRβ and CD31 expression in the prefrontal cortex. (B) Representative immunoblots of PDGFRβ, CD31, and GLUT1 expression in the hippocampus. (C) Representative immunoblots of Occludin, Claudin‐5, ZO‐1, NF‐κB, TNF‐α, and IL‐1β expression in the hippocampus. (D) Quantitative data of PDGFRβ and CD31 expression in the prefrontal cortex. (E) Quantitative data of PDGFRβ, CD31, and GLUT1 expression in the hippocampus. (F) Quantitative analysis of Occludin, Claudin‐5, and ZO‐1 expression in the hippocampus. (G) Quantitative analysis of NF‐κB, TNF‐α, and IL‐1β expression in the hippocampus. Data are expressed as mean ± SEM ( n = 3 per group). * p < 0.05 between groups. (one‐way ANOVA followed by Tukey test).

Article Snippet: After blocking with 5% non‐fat milk for 2 h at room temperature, the membranes were incubated with primary antibodies overnight at 4°C: GAPDH (1:1000, BM3874, Boster, China), platelet‐derived growth factor receptor beta (PDGFRβ) (1:1000, A00096‐1, Boster, China), Zonula occludens‐1 (ZO‐1) (1:1000, PB9234, Boster, China), Claudin 5 (1:1000, 29,767–1‐AP, Proteintech, China), Occludin (1:1000, A01246‐4, Boster, China), NF‐κB (1:1000, CY5034, Abways, China), TNF‐α (1:1000, RM8040, Biodragon, China), IL‐1β (1:1000, CY5087, Abways, China), BDNF (1:1000, E17G19, Selleck, China), PSD95 (1:1000, F17C8, Selleck, China), CD31 (1:1000, 11,265–1‐AP, Proteintech, China), glucose transporters 1 (GLUT1) (1:1000, P22E15, Selleck, China).

Techniques: Expressing, Western Blot