Journal: Scientific Reports

Article Title: The fluoroquinoline compound exerts anti-erythroleukemic effects by dual-targeting GLUT1 and the PI3K/AKT signaling pathway

doi: 10.1038/s41598-026-45597-9

Figure Lengend Snippet: FKL-137 targeting GLUT1. ( A ) Drug disease Target Venn Diagram. ( B ) Visualization analysis of the docking of FKL-137 with GLUT1. ( C , D ) Detection of GLUT1 expression after culturing K562 cells with FKL-137 using cell thermodynamics experiments. ( E , F ) The total protein levels of GLUT1 in K562 cells after treatment with FKL-137 (0.01, 0.1, 1) for 2 h were verified under non-reducing conditions. ( G ) Effects of FKL-137 on G61 glucose analogues uptake in K562 cells. G61 glucose analogues uptake was measured at various concentrations of FKL-137, ranging from 0-0.6 µM for 6 h. Uptake data ( n = 4) were normalized to basal uptake (0 μm FKL-137) at 100%. ( H ) The effect of 0.2 µM FKL-137 on the G61 glucose analogues uptake in K562 cells was investigated in different concentrations of glucose culture media. Under the condition of 0.2 µM FKL-137 for 6 h, the influence on G61 glucose analogues uptake in K562 cells was detected under the conditions of 5 mM and 30 mM glucose culture, and it was standardized against the uptake amount of the control group at 5 mM. ( I ) Evaluation of the effect of FKL-137 on GLUT1 expression by immunofluorescence staining method. The values are presented as the mean ± SD ( n = 3). ANOVA with Fisher’s LSD test was used for comparison between different groups. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. DMSO group.

Article Snippet: Erythroleukeamia cells were subjected to Immunofluorescent (IFC) staining using antibodies against GLUT1 (Red, 1:500, ET1601-10, HUABIO).

Techniques: Expressing, Analogues, Control, Immunofluorescence, Staining, Comparison

Journal: Scientific Reports

Article Title: The fluoroquinoline compound exerts anti-erythroleukemic effects by dual-targeting GLUT1 and the PI3K/AKT signaling pathway

doi: 10.1038/s41598-026-45597-9

Figure Lengend Snippet: FKL-137 targets GLUT1 to regulate the dysregulated glucose metabolism of erythroleukemia cells. ( A , B ) The protein expression level of GLUT1 in HEL and K562 cells. ( C , D ) Western blot assay to detect the expression results of GLUT1 after silencing of shGLUT1. ( E ) The MTT method was used to detect the cell proliferation of the control group and the gene silencing group. ( F , G ) The inhibitory effect of FKL-137 on the proliferation of sh GLUT1 cells. ( H ) The glucose uptake of K562 cells after glut1 knockdown was observed after treatment with FKL-137. ( I ) The the lactate secretion levels of K562 cells after glut1 knockdown was observed after treatment with FKL-137. ( J , K ) Western blot was used to detect the expression levels of glucose metabolism-related proteins in K562 cells, the control group with knockdown treatment, and K562 cells after GLUT1 knockdown. The values are presented as the mean ± SD ( n = 3). ANOVA with Fisher’s LSD test was used for comparison between different groups. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. NC group or K562 group.

Article Snippet: Erythroleukeamia cells were subjected to Immunofluorescent (IFC) staining using antibodies against GLUT1 (Red, 1:500, ET1601-10, HUABIO).

Techniques: Expressing, Western Blot, Control, Knockdown, Comparison

Journal: Scientific Reports

Article Title: The fluoroquinoline compound exerts anti-erythroleukemic effects by dual-targeting GLUT1 and the PI3K/AKT signaling pathway

doi: 10.1038/s41598-026-45597-9

Figure Lengend Snippet: FKL-137 regulates the PI3K/AKT signaling pathway in erythroleukemia cells. ( A – D ) FKL-137 (0, 0.1, 0.2 and 0.4 µmol/L) was applied to HEL and K562 cells for 48 h, and the expression changes of PI3K, P-PI3K, AKT, and P-AKT were observed. ( E ) The mechanism diagram of the anti-erythroleukemia effect of FKL-137 by targeting GLUT1 to regulate glucose metabolism. The values are presented as the mean ± SD ( n = 3). ANOVA with Fisher’s LSD test was used for comparison between different groups. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. DMSO group.

Article Snippet: Erythroleukeamia cells were subjected to Immunofluorescent (IFC) staining using antibodies against GLUT1 (Red, 1:500, ET1601-10, HUABIO).

Techniques: Expressing, Comparison